Bis-Tris is a very versatile zwitterionic biological buffer commonly used in biological and biochemical research. Bis-Tris is named according to its structure. Unlike most other available biological buffers, Bis-Tris' has functional groups associated with two distinct structural families: the bis(2-hydroxyethyl)amine family and the Tris family.
Bis-tris in Detail
Bis-Tris full name is bis (2-hydroxyethyl) amino (trimethylol) methane, CAS number: 6976-37-0, is an inert zwitterionic buffer, the effective pH buffer range is 5.8-7.2, pKa =6.5 (25°C), the structure is similar to Tris, hence the name. Bis-Tris has only a slight change in its dissociation constant with the change of temperature. It can ionize a small amount of hydrogen ions and is weakly acidic, so it is suitable for weakly acidic buffer systems.
Advantages of Using Bis-tris Gels for Protein Electrophoresis:
The gel runs at neutral pH, which minimizes protein denaturation and helps improve protein stability during electrophoresis, which in turn helps to improve band clarity and resolution;
The electrophoresis time is about 40% shorter than that of standard Tris-glycine;
The casted gel can be stored at 4°C for up to 4 weeks and is compatible with standard staining and transfer methods;
Premixed acrylamide buffer improves reproducibility while saving time;
Flexibility: Dilute the separation solution with deionized water to make its gel percentage reach any value between 8% and 15%;
The rapid glue making solution supports the simultaneous polymerization of lamination glue and separation glue, which can further save time.
Advantages of Desheng in Bis-tris:
Reliable quality, professional production base, using imported production equipment to ensure that Bis-tris can meet customer requirements in all parameters;
The after-sales service is good. After purchasing the raw materials, the customer may encounter various problems and needs to be guided by a special person. This requires the selected raw material supplier to provide the corresponding after-sales service. In this regard, Desheng is very professional;
Good reputation in the industry, which can better reflect the comprehensive strength of an enterprise. As a bis tris manufacturer, Desheng has a good reputation in the market and has been affirmed by many companies;
The manufacturer provides stable supply. Desheng has a high daily output and sufficient inventory reserves. Even if there is a large demand, Desheng can deliver the money quickly.
Applications of Bis-tris
Used as a tris bis buffer for anion exchange chromatography;
Used as electrophoresis buffer, gel buffer and sample buffer;
For agarose gel electrophoresis;
It can protect its stability in the separation of hemoglobin;
It can be used as an effective buffer for the analysis of muscle enzyme activity.
Bis-tris Notes
Forms strong complexes with Pb2+ and Cu2+, a weak interaction with other common metals;
When used with BCA detection, it will affect the use effect;
To avoid affecting protein kinetics, it is not recommended to use with FAB.
Bis-Tris forms complexes with heavy metals such as lead and copper.
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